WebJun 6, 2024 · FBS: Biowest: Cat#S1820-500: L-glutamine: GIBCO: Cat#25030-081: amino acid-free DMEM: Wako Pure Chemical Industries: Cat#048-33575: SlowFade antifade reagents: Thermo Fisher Scientific ... To obtain postnuclear supernatants, tissue homogenates were centrifuged at 500 × g for 10 min, and the supernatants were boiled … WebJun 4, 2024 · 3.1 TRIC System Allows the Visualization of Mating-Dependent Activation of Neurons 3.1.1 Preparation of Transgenic Flies. 1. Cross Tdc2-GAL4 line with TRIC line and rear their progeny at 25 °C to become adults.. 2. Collect the virgin females carrying the genotype Tdc2 > TRIC, which corresponds to w*, UAS-mCD8::RFP, LexAop2 …
Fetal Bovine Serum - FBS - Biosera
WebHEK293T cells expressing the GFP-LC3-RFP autophagy flux reporter and Cas9 (HEK293T GFP-LC3-RFP cells) were cultured in 10% fetal bovine serum (S1820-500; biowest) supplemented DMEM (D6546; Sigma-Aldrich). HEK293T GFP-LC3-RFP cells were transfected the GeCKOv2 library at a multiplicity of infection (MOI) of 0.3–0.5 and … saint andrewism youtube
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WebFetal Bovine Serum (FBS) Biowest Cat# S1820-500 Penicillin-Streptomycin Mixed Solution (PC/SM) Nacalai Tesque Cat# 09367-34 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat# L3000001 0.22-mM syringe-driven filter unit Merck Millipore Cat# SLGVR33RS NaCl Nacalai Tesque Cat# 31320-05 WebFetal Bovine Serum (FBS) South America, Tetracycline Free: The serum is tested for the presence of Chlortetracycline, Oxytetracycline and Tetracycline by a liquid chromatography electrospray ionisation tandem mass spectrometry method. The detection limit is . 0.05 mg/l. WebFetal Bovine Serum (FBS) Company: BioWest: Catalog#: S1820-500. Similar Protocol. Isolation and Separation of Epithelial CD34 + Cancer Stem Cells from Tgfbr2-deficient … thierry secretan