Webtreated with PTX, and quinpirole-induced responses were mea-sured. Infection of NS20Y-D 2L cells with HSV-Ga o* rescued both inhibition and sensitization in PTX-treated cells, whereas infection with HSV-Ga i1*, HSV-Ga i2*, or HSV-Ga i3* failed to rescue either response. In summary, the current study provides strong evidence that the D WebJan 30, 2024 · Forskolin-induced swelling of patient-derived organoids has been used to measure patient-specific CFTR function and CFTR modulator response. We present a …
Forskolin-induced Swelling in Intestinal Organoids: An …
WebMay 1, 2024 · Here we evaluate ELX-02 mediated read-through using the CFTR-dependent Forskolin-induced swelling (FIS) assay across a selection of G542X genotype patient derived organoids (PDOs). Methods. CFTR functional restoration was evaluated in ELX-02 treated G542X homozygous and heterozygous PDOs in the CFTR-dependent FIS assay. WebForskolin-Induced Swelling Assays Cystic fibrosis is caused by mutations in the CFTR gene and is highly variable between individuals, with over 1,900 CFTR mutations … how news is made
Protocol for Application, Standardization and Validation …
WebSabine Heida-Michel is an academic researcher from Utrecht University. The author has contributed to research in topic(s): Cystic fibrosis & Cystic fibrosis transmembrane conductance regulator. The author has an hindex of 2, … WebNov 14, 2024 · The CFTR-dependent forskolin-induced swelling assay in intestinal organoids can be used to estimate quantitative differences between CFTR residual function of patients. We searched on PubMed … WebDownload scientific diagram Comparison of the forskolin-induced transepithelial current (2D HIO Ieq) and the forskolin-induced swelling (3D FIS) assessment in human intestinal organoids. (A, B ... men who whine